Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
Belongs to the DOCK family. Contains 1 DHR-1 (CZH-1) domain. Contains 1 DHR-2 (CZH-2) domain. Contains 1 SH3 domain.
The DHR-2 domain is necessary and sufficient for the GEF activity.
Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
DOCK1 staining (4µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane).Primary incubated for 12 hour.Detected by western blot using chemiluminescence DOCK1 staining (4µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence