Key features and details
- Rabbit polyclonal to DOCK10
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DOCK10 antibody
DescriptionRabbit polyclonal to DOCK10
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide corresponding to a region between residue 100 and 150 of human DOCK10 (NP_055504.2)
- HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 250 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceDOCK10 is a potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Two named isoforms exist, produced by alternative splicing.
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All lanes : Anti-DOCK10 antibody (ab75258) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 250 kDa
Observed band size: 250 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Detection of DOCK10 by Western Blot of Immunprecipitate.
ab75258 at 1µg/ml staining DOCK10 in HeLa whole cell lysate immunoprecipitated using ab75258 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Detection: chemiluminescence with exposure time of 1 minute.
ab75258 has not yet been referenced specifically in any publications.