Product nameAnti-DOCK10 antibody
DescriptionRabbit polyclonal to DOCK10
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide corresponding to a region between residue 100 and 150 of human DOCK10 (NP_055504.2)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 250 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceDOCK10 is a potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Two named isoforms exist, produced by alternative splicing.
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All lanes : Anti-DOCK10 antibody (ab75258) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 250 kDa
Observed band size: 250 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Detection of DOCK10 by Western Blot of Immunprecipitate.
ab75258 at 1µg/ml staining DOCK10 in HeLa whole cell lysate immunoprecipitated using ab75258 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Detection: chemiluminescence with exposure time of 1 minute.
ab75258 has not yet been referenced specifically in any publications.