Key features and details
- Rabbit polyclonal to DOCK180
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DOCK180 antibody
See all DOCK180 primary antibodies
DescriptionRabbit polyclonal to DOCK180
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human DOCK180.
Database link: NP_001371.1
- HepG2 whole cell lysate (ab7900) can be used as a positive control in WB. HeLa, 293T and NIH3T3 whole cell lysates.
Previously labelled as DOCK1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab76927 was affinity purified using an epitope specific to DOCK180 immobilized on solid support.
Our Abpromise guarantee covers the use of ab76927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 215 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
Tissue specificityHighly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
Sequence similaritiesBelongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
Contains 1 SH3 domain.
DomainThe DHR-2 domain is necessary and sufficient for the GEF activity.
Cellular localizationCytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
- Information by UniProt
- 180 kDa protein downstream of CRK antibody
- Ced5 antibody
- Dedicator of Cytokinesis 1 antibody
All lanes : Anti-DOCK180 antibody (ab76927) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 215 kDa
Observed band size: 215 kDa
Exposure time: 3 minutes
Detection of DOCK180 by Western Blot of Immunprecipitate.
ab76927, at 1µg/ml, staining DOCK180 in HeLa whole cell lysate immunoprecipitated using ab76927 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
ab76927 has not yet been referenced specifically in any publications.