Overview

  • Product name

  • Description

    Rabbit polyclonal to DOCK180
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DOCK180 aa 1852-1865.
    Sequence:

    TTRKQASVDSGIVQ

  • Positive control

    • WB: 293T whole cell lysate; HeLaS3 cell lysate ICC/IF: A431 cells IHC-P: Lung CA
  • General notes

    Previously labelled as DOCK1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab97325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/3000. Predicted molecular weight: 215 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
  • Tissue specificity

    Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
  • Sequence similarities

    Belongs to the DOCK family.
    Contains 1 DHR-1 (CZH-1) domain.
    Contains 1 DHR-2 (CZH-2) domain.
    Contains 1 SH3 domain.
  • Domain

    The DHR-2 domain is necessary and sufficient for the GEF activity.
  • Cellular localization

    Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
  • Information by UniProt
  • Database links

  • Alternative names

    • 180 kDa protein downstream of CRK antibody
    • Ced5 antibody
    • Dedicator of Cytokinesis 1 antibody
    • Dedicator of cytokinesis protein 1 antibody
    • DOCK 1 antibody
    • DOCK 180 antibody
    • DOCK1 antibody
    • DOCK1_HUMAN antibody
    • DOCK180 antibody
    see all

Images

  • Anti-DOCK180 antibody (ab97325) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 215 kDa



    7.5% SDS Page
  • Immunofluorescence analysis of DOCK180 in methanol fixed A431, using ab97325 at a 1/200 dilution.

  • Immunohistochemical analysis of DOCK180 in paraffin embedded Lung CA, using ab97325 at a 1/100 dilution.

References

This product has been referenced in:

  • Liang Y  et al. Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple-negative breast cancer epithelial cells through the RhoA/Rac1 pathway. Oncol Lett 16:3481-3488 (2018). Read more (PubMed: 30127952) »
  • Toret CP  et al. An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion. J Cell Biol 207:577-87 (2014). Read more (PubMed: 25452388) »
See all 2 Publications for this product

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