Anti-DOCK2 antibody - C-terminal (ab226797)
Key features and details
- Rabbit polyclonal to DOCK2 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOCK2 antibody - C-terminal
See all DOCK2 primary antibodies -
Description
Rabbit polyclonal to DOCK2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment within Human DOCK2 (C terminal). The exact sequence is proprietary.
Database link: Q92608 -
Positive control
- ICC/IF: Jurkat cells. WB: Jurkat, Raji and NCI-H929 whole cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 212 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 212 kDa. |
Target
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Relevance
DOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2. -
Cellular localization
Intracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin. -
Database links
- Entrez Gene: 1794 Human
- Entrez Gene: 94176 Mouse
- Omim: 603122 Human
- SwissProt: Q92608 Human
- SwissProt: Q8C3J5 Mouse
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Alternative names
- Dedicator of cyto kinesis 2 antibody
- Dedicator of cytokinesis 2 antibody
- Dedicator of cytokinesis protein 2 antibody
see all
Images
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Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for DOCK2 (green) using ab226797 at 1/400 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.
Blue: Hoechst 33342 staining.
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All lanes : Anti-DOCK2 antibody - C-terminal (ab226797) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 212 kDa5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226797 has not yet been referenced specifically in any publications.