Key features and details
- Rabbit polyclonal to DOCK2 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DOCK2 antibody - C-terminal
See all DOCK2 primary antibodies
DescriptionRabbit polyclonal to DOCK2 - C-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment within Human DOCK2 (C terminal). The exact sequence is proprietary.
Database link: Q92608
- ICC/IF: Jurkat cells. WB: Jurkat, Raji and NCI-H929 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 212 kDa.|
RelevanceDOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2.
Cellular localizationIntracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin.
- Dedicator of cyto kinesis 2 antibody
- Dedicator of cytokinesis 2 antibody
- Dedicator of cytokinesis protein 2 antibody
Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for DOCK2 (green) using ab226797 at 1/400 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.
Blue: Hoechst 33342 staining.
All lanes : Anti-DOCK2 antibody - C-terminal (ab226797) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 212 kDa
5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226797 has not yet been referenced specifically in any publications.