Overview

  • Product name

    Anti-DOCK2 antibody - C-terminal
    See all DOCK2 primary antibodies
  • Description

    Rabbit polyclonal to DOCK2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment within Human DOCK2 (C terminal). The exact sequence is proprietary.
    Database link: Q92608

  • Positive control

    • ICC/IF: Jurkat cells. WB: Jurkat, Raji and NCI-H929 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab226797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 212 kDa.

Target

  • Relevance

    DOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2.
  • Cellular localization

    Intracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin.
  • Database links

  • Alternative names

    • Dedicator of cyto kinesis 2 antibody
    • Dedicator of cytokinesis 2 antibody
    • Dedicator of cytokinesis protein 2 antibody
    • DOCK 2 antibody
    • FLJ46592 antibody
    • IMD40 antibody
    • KIAA0209 antibody
    see all

Images

  • Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for DOCK2 (green) using ab226797 at 1/400 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-DOCK2 antibody - C-terminal (ab226797) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 212 kDa



    5% SDS-PAGE gel.

References

ab226797 has not yet been referenced specifically in any publications.

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