RecombinantRabMAb

Recombinant Anti-DOCK2 antibody [EPR6647] (ab124838)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6647] to DOCK2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-DOCK2 antibody [EPR6647]
    See all DOCK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6647] to DOCK2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human DOCK2 protein (Q92608).

  • Positive control

    • Jurkat and Raji cell lysates and Human lung tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab124838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 212 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is not recommended.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Relevance

      DOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2.

    • Cellular localization

      Intracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin.

    • Database links

      • Alternative names

        • Dedicator of cyto kinesis 2 antibody
        • Dedicator of cytokinesis 2 antibody
        • Dedicator of cytokinesis protein 2 antibody
        • DOCK 2 antibody
        • FLJ46592 antibody
        • IMD40 antibody
        • KIAA0209 antibody
        see all

      Images

      • Western blot - Anti-DOCK2 antibody [EPR6647] (ab124838)
        Western blot - Anti-DOCK2 antibody [EPR6647] (ab124838)
        All lanes : Anti-DOCK2 antibody [EPR6647] (ab124838) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : Raji cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 212 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK2 antibody [EPR6647] (ab124838)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK2 antibody [EPR6647] (ab124838)

        ab124838, at 1/50 - 1/100, staining DOCK2 in Human lung tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-DOCK2 antibody [EPR6647] (ab124838)
        Anti-DOCK2 antibody [EPR6647] (ab124838)

      References (1)

      Publishing research using ab124838? Please let us know so that we can cite the reference in this datasheet.

      ab124838 has been referenced in 1 publication.

      • Cao M  et al. Mechanisms of Impaired Neutrophil Migration by MicroRNAs in Myelodysplastic Syndromes. J Immunol 198:1887-1899 (2017). PubMed: 28130497

      Customer reviews and Q&As

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      Question

      I would like to ask about the IHC staining of the formalin-fixed, paraffin-embeded tissue sections with the Anti-DOCK2 antibody [EPR6647] (ab124838). What type of the antigen retrieval solution is recommended (the manual says that none, but I would like to confirm it). If no antigen retrieval solution is recommended, does this step of the protocol should be completely skipped?

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      Answer

      You are correct, we observed better staining in IHC-P with ab124838 when no antigen retrieval was used. Thus, we recommend skipping the antigen retrieval step in your protocol. This may vary depending on your tissue type and processing methods, so it may still be worthwhile in your initial optimization testing our no antigen retrieval with a standard heat mediated antigen retrieval side by side to see which gives better staining in your specific tissue samples.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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