
Recombinant Anti-DOCK2 antibody [EPR6647] (ab124838)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-DOCK2 antibody [EPR6647]
See all DOCK2 primary antibodies -
Description
Rabbit monoclonal [EPR6647] to DOCK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of Human DOCK2 protein (Q92608).
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Positive control
- Jurkat and Raji cell lysates and Human lung tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Purification notes
Not purified; provided in cell supernatant and storage buffer. -
Clonality
Monoclonal -
Clone number
EPR6647 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Predicted molecular weight: 212 kDa. | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is not recommended. |
Target
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Relevance
DOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2. -
Cellular localization
Intracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin. -
Database links
- Entrez Gene: 1794 Human
- Omim: 603122 Human
- SwissProt: Q92608 Human
- Unigene: 586174 Human
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Alternative names
- Dedicator of cyto kinesis 2 antibody
- Dedicator of cytokinesis 2 antibody
- Dedicator of cytokinesis protein 2 antibody
see all
Images
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All lanes : Anti-DOCK2 antibody [EPR6647] (ab124838) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 212 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOCK2 antibody [EPR6647] (ab124838)
ab124838, at 1/50 - 1/100, staining DOCK2 in Human lung tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Cao M et al. Mechanisms of Impaired Neutrophil Migration by MicroRNAs in Myelodysplastic Syndromes. J Immunol 198:1887-1899 (2017). Read more (PubMed: 28130497) »