Product nameAnti-DOCK4 antibody
DescriptionRabbit polyclonal to DOCK4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 1725-1775 of Human DOCK4 NP_055520.3
- HeLa whole cell lysate (ab150035), 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 225 kDa.|
|IP||Use at 3-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in regulation of adherens junction between cells. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.
Tissue specificityWidely expressed at low level. Highly expressed in skeletal muscle, prostate and ovary.
Sequence similaritiesBelongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
Contains 1 SH3 domain.
DomainThe DHR-2 domain probably mediates the GEF activity.
Cellular localizationEndomembrane system.
- Information by UniProt
- Dedicator of cytokinesis protein 4 antibody
- Dock4 antibody
- DOCK4_HUMAN antibody
- KIAA0716 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DOCK4 with ab85723 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-DOCK4 antibody (ab85723) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 225 kDa
Observed band size: 225 kDa
Exposure time: 30 seconds
Detection of Human DOCK4 by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded)using ab85723 at 3ug/mg lysate. Subsequent Western blot detection was performed using 0.4 ug/ml ab85723. Detection: Chemiluminescence with exposure times of 30 seconds.
This product has been referenced in:
- Guo D et al. Autism-like social deficit generated by Dock4 deficiency is rescued by restoration of Rac1 activity and NMDA receptor function. Mol Psychiatry N/A:N/A (2019). Read more (PubMed: 31388105) »
- Frank SR et al. p190 RhoGAP promotes contact inhibition in epithelial cells by repressing YAP activity. J Cell Biol 217:3183-3201 (2018). Read more (PubMed: 29934311) »