Key features and details
- Mouse monoclonal [3540C1a] to DOCK7
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Product nameAnti-DOCK7 antibody [3540C1a]
See all DOCK7 primary antibodies
DescriptionMouse monoclonal [3540C1a] to DOCK7
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Human
Recombinant fragment with a tag, corresponding to internal sequence amino acids 1003-1112 of Human DOCK7
- Immunising recombinant Human DOCK7 protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesab84866 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab84866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 243 kDa.|
RelevanceDOCK7 functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. It does not have a GEF activity for CDC42. It is required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization.
Cellular localizationCell projection, axon. Note=Enriched in the developing axons of hippocampal neurons.
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Anti-DOCK7 antibody [3540C1a] (ab84866) + Immunogen (Recombinant Human DOCK7 fragment)
Predicted band size: 243 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab84866 has not yet been referenced specifically in any publications.