Anti-DOCK7 antibody (ab118790)
Key features and details
- Rabbit polyclonal to DOCK7
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOCK7 antibody
See all DOCK7 primary antibodies -
Description
Rabbit polyclonal to DOCK7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to Human DOCK7 aa 150-250.
Database link: Q96N67 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab118790 was affinity purified using an epitope specific to DOCK7 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 243 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Relevance
DOCK7 functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. It does not have a GEF activity for CDC42. It is required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization. -
Cellular localization
Cell projection, axon. Note=Enriched in the developing axons of hippocampal neurons. -
Database links
- Entrez Gene: 85440 Human
- GenBank: BC016392.1 Human
- SwissProt: Q96N67 Human
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Alternative names
- 3110056M06Rik antibody
- dedicator of cytokinesis 7 antibody
- Dedicator of cytokinesis protein 7 antibody
see all
Images
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All lanes : Anti-DOCK7 antibody (ab118790) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 243 kDa
Exposure time: 30 seconds -
Detection of DOCK7 in Immunoprecipitates of HeLa whole cell lysatse (1 mg for IP, 20% of IP loaded) using ab118790 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab118790 has been referenced in 2 publications.
- Le Couteur DG et al. Nutritional reprogramming of mouse liver proteome is dampened by metformin, resveratrol, and rapamycin. Cell Metab 33:2367-2379.e4 (2021). PubMed: 34767745
- Nakamuta S et al. Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain. J Cell Biol 216:4313-4330 (2017). PubMed: 29089377