Anti-DOCK8 antibody (ab227529)
Key features and details
- Rabbit polyclonal to DOCK8
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-DOCK8 antibody
See all DOCK8 primary antibodies -
Description
Rabbit polyclonal to DOCK8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Synthetic peptide within Human DOCK8 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q8NF50 -
Positive control
- WB: Raji and NCI-H929 whole cell extracts. IHC-P: Mouse lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 239 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 239 kDa. |
Target
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Function
Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. -
Involvement in disease
Defects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES) [MIM:243700]. It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement. -
Sequence similarities
Belongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain. -
Domain
The DHR-2 domain may mediate some GEF activity. - Information by UniProt
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Database links
- Entrez Gene: 81704 Human
- Entrez Gene: 76088 Mouse
- Entrez Gene: 499337 Rat
- Omim: 611432 Human
- SwissProt: Q8NF50 Human
- SwissProt: Q8C147 Mouse
- Unigene: 132599 Human
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Alternative names
- 1200017A24Rik antibody
- Dedicator of cytokinesis protein 8 antibody
- DOCK8 antibody
see all
Images
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All lanes : Anti-DOCK8 antibody (ab227529) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 2 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 239 kDa5% SDS-PAGE
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Paraffin-embedded mouse lung tissue stained for DOCK8 with ab227529 (1/500 dilution) in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227529 has not yet been referenced specifically in any publications.