Product nameAnti-DOCK8 antibody
See all DOCK8 primary antibodies
DescriptionRabbit polyclonal to DOCK8
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide within Human DOCK8 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q8NF50
- WB: Raji and NCI-H929 whole cell extracts. IHC-P: Mouse lung tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 239 kDa.|
FunctionPotential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP.
Involvement in diseaseDefects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES) [MIM:243700]. It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement.
Sequence similaritiesBelongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
DomainThe DHR-2 domain may mediate some GEF activity.
- Information by UniProt
- 1200017A24Rik antibody
- Dedicator of cytokinesis protein 8 antibody
- DOCK8 antibody
All lanes : Anti-DOCK8 antibody (ab227529) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 2 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 239 kDa
Paraffin-embedded mouse lung tissue stained for DOCK8 with ab227529 (1/500 dilution) in immunohistochemical analysis.
ab227529 has not yet been referenced specifically in any publications.