Recombinant
RabMAb

Recombinant Anti-DOCK8 antibody [EPR12511-56] (ab178427)

Overview

  • Product name

    Anti-DOCK8 antibody [EPR12511-56]
    See all DOCK8 primary antibodies
  • Description

    Rabbit monoclonal [EPR12511-56] to DOCK8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human DOCK8. The exact sequence is proprietary.
    Database link: Q8NF50

  • Positive control

    • THP1, Raji and Ramos cell line lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12511-56
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 239 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC or IP.
  • Target

    • Function

      Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP.
    • Involvement in disease

      Defects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES) [MIM:243700]. It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement.
    • Sequence similarities

      Belongs to the DOCK family.
      Contains 1 DHR-1 (CZH-1) domain.
      Contains 1 DHR-2 (CZH-2) domain.
    • Domain

      The DHR-2 domain may mediate some GEF activity.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1200017A24Rik antibody
      • Dedicator of cytokinesis protein 8 antibody
      • DOCK8 antibody
      • DOCK8_HUMAN antibody
      • Epididymis luminal protein 205 antibody
      • HEL 205 antibody
      • MRD2 antibody
      • ZIR8 antibody
      see all

    Images

    • All lanes : Anti-DOCK8 antibody [EPR12511-56] (ab178427) at 1/1000 dilution

      Lane 1 : THP1 cell line lysate
      Lane 2 : Raji cell line lysate
      Lane 3 : Ramos cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody

      Developed using the ECL technique.

      Predicted band size: 239 kDa

    References

    ab178427 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab178427.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up