Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12511-56] to DOCK8
- Suitable for: WB
- Reacts with: Human
Product nameAnti-DOCK8 antibody [EPR12511-56]
See all DOCK8 primary antibodies
DescriptionRabbit monoclonal [EPR12511-56] to DOCK8
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC or IP
Species reactivityReacts with: Human
Recombinant fragment within Human DOCK8. The exact sequence is proprietary.
Database link: Q8NF50
- THP1, Raji and Ramos cell line lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 239 kDa.|
FunctionPotential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP.
Involvement in diseaseDefects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES) [MIM:243700]. It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement.
Sequence similaritiesBelongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
DomainThe DHR-2 domain may mediate some GEF activity.
- Information by UniProt
- 1200017A24Rik antibody
- Dedicator of cytokinesis protein 8 antibody
- DOCK8 antibody
All lanes : Anti-DOCK8 antibody [EPR12511-56] (ab178427) at 1/1000 dilution
Lane 1 : THP1 cell line lysate
Lane 2 : Raji cell line lysate
Lane 3 : Ramos cell line lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody
Developed using the ECL technique.
Predicted band size: 239 kDa
ab178427 has not yet been referenced specifically in any publications.