Product nameAnti-DOCK8 antibody [EPR12511]
See all DOCK8 primary antibodies
DescriptionRabbit monoclonal [EPR12511] to DOCK8
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Recombinant fragment within Human DOCK8. The exact sequence is proprietary.
Database link: Q8NF50
- Raji and THP1 cell lysate
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 239 kDa.|
For unpurified use at 1/100 - 1/250
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPotential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP.
Involvement in diseaseDefects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES) [MIM:243700]. It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement.
Sequence similaritiesBelongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
DomainThe DHR-2 domain may mediate some GEF activity.
- Information by UniProt
- 1200017A24Rik antibody
- Dedicator of cytokinesis protein 8 antibody
- DOCK8 antibody
Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
All lanes : Anti-DOCK8 antibody [EPR12511] (ab175208) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 239 kDa
Immunofluorescent staining of Raji cells labeling DOCK8 with ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized THP1 cells labeling DOCK8 with ab175208 at 1/10 dilution (red) compared to a Rabbit IgG (green).
This product has been referenced in:
- Meshaal SS et al. Diagnosis of DOCK8 deficiency using Flow cytometry Biomarkers: an Egyptian Center experience. Clin Immunol 195:36-44 (2018). Read more (PubMed: 30048691) »
- Qin T et al. Novel DOCK8 gene mutations lead to absence of protein expression in patients with hyper-IgE syndrome. Immunol Res N/A:N/A (2015). Read more (PubMed: 26659092) »