Anti-DOCK9/Trg antibody (ab204421)
Key features and details
- Rabbit polyclonal to DOCK9/Trg
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOCK9/Trg antibody
See all DOCK9/Trg primary antibodies -
Description
Rabbit polyclonal to DOCK9/Trg -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human DOCK9/Trg aa 250-400.
Database link: Q9BZ29 -
Positive control
- IHC-P: Human cerebellum, placenta, lung tissue. ICC/IF: A431 cells. WB: Caco-2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 236 kDa.
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Notes |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 236 kDa. |
Target
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Function
Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation. -
Tissue specificity
Widely expressed, with highest expression in heart and placenta. Expressed at intermediate level in kidney, brain, lung and skeletal muscle. -
Sequence similarities
Belongs to the DOCK family.
Contains 1 DHR-1 domain.
Contains 1 DHR-2 domain.
Contains 1 PH domain. -
Domain
The DHR-2 domain is necessary and sufficient for the GEF activity. -
Cellular localization
Endomembrane system. Associated with membranes. - Information by UniProt
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Database links
- Entrez Gene: 23348 Human
- Entrez Gene: 105445 Mouse
- Entrez Gene: 259237 Rat
- Omim: 607325 Human
- SwissProt: Q9BZ29 Human
- SwissProt: Q8BIK4 Mouse
- SwissProt: Q63603 Rat
- Unigene: 596105 Human
see all -
Alternative names
- Cdc42 guanine nucleotide exchange factor zizimin 1 antibody
- Cdc42 guanine nucleotide exchange factor zizimin-1 antibody
- Dedicator of cytokinesis protein 9 antibody
see all
Images
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Lane 2 : Anti-DOCK9/Trg antibody (ab204421) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Caco-2 cell lysate
Predicted band size: 236 kDa -
Paraffin embedded human placenta tissue stained for DOCK9/Trg using ab204421 at 1/1000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human cerebellum tissue stained for DOCK9/Trg using ab204421 at 1/1000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human lung tissue stained for DOCK9/Trg using ab204421 at 1/1000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human pancreas tissue stained for DOCK9/Trg using ab204421 at 1/1000 dilution in immunohistochemical analysis. Shows no positivity in exocrine glandular cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204421 has not yet been referenced specifically in any publications.