Key features and details
- Rabbit polyclonal to DOCK9/Trg
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DOCK9/Trg antibody
See all DOCK9/Trg primary antibodies
DescriptionRabbit polyclonal to DOCK9/Trg
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Opossum, Orangutan
Synthetic peptide, corresponding to amino acids 1-50 of Human DOCK9 (NP_056111.1)
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/5000 - 1/15000. Detects a band of approximately 236 kDa (predicted molecular weight: 236 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionGuanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.
Tissue specificityWidely expressed, with highest expression in heart and placenta. Expressed at intermediate level in kidney, brain, lung and skeletal muscle.
Sequence similaritiesBelongs to the DOCK family.
Contains 1 DHR-1 domain.
Contains 1 DHR-2 domain.
Contains 1 PH domain.
DomainThe DHR-2 domain is necessary and sufficient for the GEF activity.
Cellular localizationEndomembrane system. Associated with membranes.
- Information by UniProt
- Cdc42 guanine nucleotide exchange factor zizimin 1 antibody
- Cdc42 guanine nucleotide exchange factor zizimin-1 antibody
- Dedicator of cytokinesis protein 9 antibody
All lanes : Anti-DOCK9/Trg antibody (ab70272) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 236 kDa
Observed band size: 236 kDa
Additional bands at: 117 kDa, 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
HeLa whole cell lysate (1 mg) was immunoprecipitated with ab70272 at 3 µg/mg of lysate (lane 1) or with IgG control (lane 2). Samples were analysed by Western blotting using ab70272 at 0.04 µg/ml and detected by chemiluminescence with a 3 minute exposure time.
ICC/IF image of ab70272 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70272, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab70272 has been referenced in 1 publication.
- Wieczfinska J et al. The whole-genome expression analysis of peripheral blood mononuclear cells from aspirin sensitive asthmatics versus aspirin tolerant patients and healthy donors after in vitro aspirin challenge. Respir Res 16:147 (2015). WB . PubMed: 26646719