Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16730] to DOHH
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-DOHH antibody [EPR16730]
See all DOHH primary antibodies
DescriptionRabbit monoclonal [EPR16730] to DOHH
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DOHH aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BU89
- WB: LNCaP, U-87 MG and SH-SY5Y whole cell lysates. IP: SH-SY5Y whole cell extract.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab197587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
FunctionCatalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
PathwayProtein modification; eIF5A hypusination.
Sequence similaritiesBelongs to the deoxyhypusine hydroxylase family.
Contains 5 HEAT-like PBS-type repeats.
- Information by UniProt
- Deoxyhypusine dioxygenase antibody
- Deoxyhypusine hydroxylase antibody
- Deoxyhypusine hydroxylase/monooxygenase antibody
All lanes : Anti-DOHH antibody [EPR16730] (ab197587) at 1/5000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 3 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 1 minute
Blocking/Dilution buffer: 5% NFDM/TBST.
DOHH was immunoprecipitated from SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell extract with ab197587 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197587 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: SH-SY5Y whole cell extract 10 µg (Input). Lane 2: ab197587 IP in SH-SY5Y whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197587 in SH-SY5Y whole cell extract
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
ab197587 has not yet been referenced specifically in any publications.