Overview

  • Product name

    Anti-DOHH antibody [EPR16730]
    See all DOHH primary antibodies
  • Description

    Rabbit monoclonal [EPR16730] to DOHH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DOHH aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BU89

  • Positive control

    • WB: LNCaP, U-87 MG and SH-SY5Y whole cell lysates. IP: SH-SY5Y whole cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/50.

Target

  • Function

    Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
  • Pathway

    Protein modification; eIF5A hypusination.
  • Sequence similarities

    Belongs to the deoxyhypusine hydroxylase family.
    Contains 5 HEAT-like PBS-type repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deoxyhypusine dioxygenase antibody
    • Deoxyhypusine hydroxylase antibody
    • Deoxyhypusine hydroxylase/monooxygenase antibody
    • Deoxyhypusine monooxygenase antibody
    • Dohh antibody
    • DOHH_HUMAN antibody
    • hDOHH antibody
    • HEAT like (PBS lyase) repeat containing 1 antibody
    • HEAT like PBS lyase repeat containing antibody
    • HEAT like repeat containing protein 1 antibody
    • HEAT-like repeat-containing protein 1 antibody
    • HLRC1 antibody
    • MGC4293 antibody
    see all

Images

  • All lanes : Anti-DOHH antibody [EPR16730] (ab197587) at 1/5000 dilution

    Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
    Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 3 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • DOHH was immunoprecipitated from SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell extract with ab197587 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197587 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: SH-SY5Y whole cell extract 10 µg (Input). Lane 2: ab197587 IP in SH-SY5Y whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197587 in SH-SY5Y whole cell extract

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab197587 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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