RecombinantRabMAb

Recombinant Anti-DOK1 antibody [EPR3228(2)] (ab155947)

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Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
  • Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
  • Anti-DOK1 antibody [EPR3228(2)] (ab155947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3228(2)] to DOK1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-DOK1 antibody [EPR3228(2)]
    See all DOK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3228(2)] to DOK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to residues in Human DOK1 (UniProt Q99704).

  • Positive control

    • HuT-78, K562 and Jurkat cell lysates; K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

    • Tissue specificity

      Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.

    • Sequence similarities

      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • Domain

      The PTB domain mediates receptor interaction.

    • Post-translational
      modifications

      Constitutively tyrosine-phosphorylated.
      Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.

    • Cellular localization

      Cytoplasm and Cytoplasm > perinuclear region.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Docking protein 1 62kD antibody
      • Docking protein 1 antibody
      • DOK 1 antibody
      • DOK1 antibody
      • DOK1_HUMAN antibody
      • Downstream of tyrosine kinase 1 antibody
      • p62(dok) antibody
      • P62DOK antibody
      • pp62 antibody
      see all

    Images

    • Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      All lanes : Anti-DOK1 antibody [EPR3228(2)] (ab155947) at 1/1000 dilution

      Lane 1 : HuT-78 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Immunofluorescent analysis of K562 cells labeling DOK1 with ab155947 at 1/250 dilution.
    • Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Anti-DOK1 antibody [EPR3228(2)] (ab155947)

    References (0)

    Publishing research using ab155947? Please let us know so that we can cite the reference in this datasheet.

    ab155947 has not yet been referenced specifically in any publications.

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