Recombinant
Anti-DOK1 antibody [EPR3228(2)] (ab155947)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-DOK1 antibody [EPR3228(2)]
See all DOK1 primary antibodies -
DescriptionRabbit monoclonal [EPR3228(2)] to DOK1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to residues in Human DOK1 (UniProt Q99704).
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Positive control
- HuT-78, K562 and Jurkat cell lysates; K562 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3228(2)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 52 kDa. | |
| ICC/IF | 1/250 - 1/500. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
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Tissue specificityExpressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
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Sequence similaritiesBelongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
DomainThe PTB domain mediates receptor interaction.
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Post-translational
modificationsConstitutively tyrosine-phosphorylated.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. -
Cellular localizationCytoplasm and Cytoplasm > perinuclear region.
- Information by UniProt
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Database links
- Entrez Gene: 1796 Human
- Entrez Gene: 13448 Mouse
- Entrez Gene: 312477 Rat
- Omim: 602919 Human
- SwissProt: Q99704 Human
- SwissProt: P97465 Mouse
- SwissProt: Q4QQV2 Rat
- Unigene: 103854 Human
see all -
Alternative names
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
see all
Images
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![Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)](https://www.abcam.com/ps/datasheet/images/155/ab155947/DOK1-Primary-antibodies-ab155947-3.jpg)
Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)All lanes : Anti-DOK1 antibody [EPR3228(2)] (ab155947) at 1/1000 dilution
Lane 1 : HuT-78 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)](https://www.abcam.com/ps/datasheet/images/155/ab155947/DOK1-Primary-antibodies-ab155947-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)Immunofluorescent analysis of K562 cells labeling DOK1 with ab155947 at 1/250 dilution.
Datasheets and documents
References
ab155947 has not yet been referenced specifically in any publications.
