Recombinant
RabMAb

Recombinant Anti-DOK1 antibody [EPR3228(2)] (ab155947)

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Overview

  • Product name

    Anti-DOK1 antibody [EPR3228(2)]
    See all DOK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3228(2)] to DOK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human DOK1 (UniProt Q99704).

  • Positive control

    • HuT-78, K562 and Jurkat cell lysates; K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab155947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

    • Tissue specificity

      Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.

    • Sequence similarities

      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • Domain

      The PTB domain mediates receptor interaction.

    • Post-translational
      modifications

      Constitutively tyrosine-phosphorylated.
      Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.

    • Cellular localization

      Cytoplasm and Cytoplasm > perinuclear region.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Docking protein 1 62kD antibody
      • Docking protein 1 antibody
      • DOK 1 antibody
      • DOK1 antibody
      • DOK1_HUMAN antibody
      • Downstream of tyrosine kinase 1 antibody
      • p62(dok) antibody
      • P62DOK antibody
      • pp62 antibody
      see all

    Images

    • Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      All lanes : Anti-DOK1 antibody [EPR3228(2)] (ab155947) at 1/1000 dilution

      Lane 1 : HuT-78 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Immunofluorescent analysis of K562 cells labeling DOK1 with ab155947 at 1/250 dilution.

    References

    ab155947 has not yet been referenced specifically in any publications.

    Publishing research using ab155947? Please let us know so that we can cite the reference in this datasheet.

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