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RecombinantRabMAb

Recombinant Anti-DOK1 antibody [EPR3228(2)] (ab155947)

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Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
  • Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
  • Anti-DOK1 antibody [EPR3228(2)] (ab155947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3228(2)] to DOK1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Protein
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Recombinant Human DOK1 protein (ab112280)
Secondary
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Overview

  • Product name

    Anti-DOK1 antibody [EPR3228(2)]
    See all DOK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3228(2)] to DOK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human DOK1 (UniProt Q99704).

  • Positive control

    • HuT-78, K562 and Jurkat cell lysates; K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3228(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Other
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-DOK1 antibody [EPR3228(2)] - BSA and Azide free (ab249202)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human DOK1 protein (ab112280)
  • Related Products

    • Recombinant Human DOK1 protein (ab112280)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
    • Tissue specificity

      Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
    • Sequence similarities

      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Domain

      The PTB domain mediates receptor interaction.
    • Post-translational
      modifications

      Constitutively tyrosine-phosphorylated.
      Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
    • Cellular localization

      Cytoplasm and Cytoplasm > perinuclear region.
    • Target information above from: UniProt accession Q99704 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1796 Human
      • Entrez Gene: 13448 Mouse
      • Entrez Gene: 312477 Rat
      • Omim: 602919 Human
      • SwissProt: Q99704 Human
      • SwissProt: P97465 Mouse
      • SwissProt: Q4QQV2 Rat
      • Unigene: 103854 Human
      • Unigene: 156 Mouse
      • Unigene: 117974 Rat
      see all
    • Alternative names

      • Docking protein 1 62kD antibody
      • Docking protein 1 antibody
      • DOK 1 antibody
      • DOK1 antibody
      • DOK1_HUMAN antibody
      • Downstream of tyrosine kinase 1 antibody
      • p62(dok) antibody
      • P62DOK antibody
      • pp62 antibody
      see all

    Images

    • Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Western blot - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      All lanes : Anti-DOK1 antibody [EPR3228(2)] (ab155947) at 1/1000 dilution

      Lane 1 : HuT-78 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Immunofluorescent analysis of K562 cells labeling DOK1 with ab155947 at 1/250 dilution.
    • Anti-DOK1 antibody [EPR3228(2)] (ab155947)
      Anti-DOK1 antibody [EPR3228(2)] (ab155947)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab155947? Please let us know so that we can cite the reference in this datasheet.

    ab155947 has not yet been referenced specifically in any publications.

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