Recombinant Anti-DOK1 antibody [EPR3228(2)] (ab155947)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3228(2)] to DOK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-DOK1 antibody [EPR3228(2)]
See all DOK1 primary antibodies -
Description
Rabbit monoclonal [EPR3228(2)] to DOK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to residues in Human DOK1 (UniProt Q99704).
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Positive control
- HuT-78, K562 and Jurkat cell lysates; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3228(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. -
Tissue specificity
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. -
Sequence similarities
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Domain
The PTB domain mediates receptor interaction. -
Post-translational
modificationsConstitutively tyrosine-phosphorylated.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. -
Cellular localization
Cytoplasm and Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 1796 Human
- Entrez Gene: 13448 Mouse
- Entrez Gene: 312477 Rat
- Omim: 602919 Human
- SwissProt: Q99704 Human
- SwissProt: P97465 Mouse
- SwissProt: Q4QQV2 Rat
- Unigene: 103854 Human
see all -
Alternative names
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
see all
Images
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All lanes : Anti-DOK1 antibody [EPR3228(2)] (ab155947) at 1/1000 dilution
Lane 1 : HuT-78 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa -
Immunofluorescent analysis of K562 cells labeling DOK1 with ab155947 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155947 has not yet been referenced specifically in any publications.