Key features and details
- Rabbit polyclonal to DOK1 (phospho Y362)
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DOK1 (phospho Y362) antibody
See all DOK1 primary antibodies
DescriptionRabbit polyclonal to DOK1 (phospho Y362)
SpecificityDetects endogenous levels of p62Dok only when phosphorylated at tyrosine 362.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesised phosphopeptide derived from p62Dok around the phosphorylation site of tyrosine 362 (Human).
- Jurkat cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab47374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).|
FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Tissue specificityExpressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
Sequence similaritiesBelongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
DomainThe PTB domain mediates receptor interaction.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
Cellular localizationCytoplasm and Cytoplasm > perinuclear region.
- Information by UniProt
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
ab47374 has not yet been referenced specifically in any publications.