Anti-DOK1 (phospho Y362) antibody (ab75742)
Key features and details
- Rabbit polyclonal to DOK1 (phospho Y362)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOK1 (phospho Y362) antibody
See all DOK1 primary antibodies -
Description
Rabbit polyclonal to DOK1 (phospho Y362) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human DOK1 (phospho Y362).
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Positive control
- Human breast carcinoma tissue and extracts from Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab75742 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. -
Tissue specificity
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. -
Sequence similarities
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Domain
The PTB domain mediates receptor interaction. -
Post-translational
modificationsConstitutively tyrosine-phosphorylated.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. -
Cellular localization
Cytoplasm and Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 1796 Human
- Omim: 602919 Human
- SwissProt: Q99704 Human
- Unigene: 103854 Human
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Alternative names
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
see all
Images
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ab75742, at a 1/50 dilution, staining DOK1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing phospho peptide.
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All lanes : Anti-DOK1 (phospho Y362) antibody (ab75742) at 1/500 dilution
Lane 1 : Jurkat cell extracts with immunising peptide
Lane 2 : Jurkat cell extracts
Predicted band size: 52 kDa
Observed band size: 52 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab75742 has been referenced in 1 publication.
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349