Key features and details
- Rabbit polyclonal to DOK1 (phospho Y398)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DOK1 (phospho Y398) antibody
See all DOK1 primary antibodies
DescriptionRabbit polyclonal to DOK1 (phospho Y398)
Specificityab75741 detects endogenous levels of DOK1 only when phosphorylated at tyrosine 398.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).
- Human breast carcinoma tissue. Extracts from K562 cells, starved.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75741 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab75741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Tissue specificityExpressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
Sequence similaritiesBelongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
DomainThe PTB domain mediates receptor interaction.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
Cellular localizationCytoplasm and Cytoplasm > perinuclear region.
- Information by UniProt
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
All lanes : Anti-DOK1 (phospho Y398) antibody (ab75741) at 1/500 dilution
Lane 1 : K562 cell extracts, unstarved
Lane 2 : K562 cell extracts, starved
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
ab75741 has not yet been referenced specifically in any publications.