Overview

  • Product name

  • Description

    Rabbit polyclonal to DOK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding internal sequence amino acids 297-301 (G-E-Y-A-V) of Human DOK2 (NP_003965.2).

  • Positive control

    • Jurkat cell extract; HeLa cells; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
  • Tissue specificity

    Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
  • Sequence similarities

    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain

    PTB domain mediates receptor interaction.
  • Post-translational
    modifications

    On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
  • Information by UniProt
  • Database links

  • Alternative names

    • Docking protein 2 56kDa antibody
    • Docking protein 2 antibody
    • DOK 2 antibody
    • DOK R antibody
    • DOK2 antibody
    • DOK2_HUMAN antibody
    • Downstream of tyrosine kinase 2 antibody
    • OTTHUMP00000161710 antibody
    • OTTHUMP00000224923 antibody
    • p56(dok 2) antibody
    • p56(dok-2) antibody
    • p56dok 2 antibody
    • p56DOK antibody
    • p56dok2 antibody
    see all

Images

  • Anti-DOK2 antibody (ab131488) at 1/500 dilution + Jurkat cell extract

    Predicted band size: 45 kDa

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling DOK2 with ab131488 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling DOK2 with ab131488 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

References

ab131488 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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