Anti-DOK2 antibody (ab131488)
Key features and details
- Rabbit polyclonal to DOK2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOK2 antibody
See all DOK2 primary antibodies -
Description
Rabbit polyclonal to DOK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, surrounding internal sequence amino acids 297-301 (G-E-Y-A-V) of Human DOK2 (NP_003965.2).
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Positive control
- Jurkat cell extract; HeLa cells; Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131488 was purified by affinity chromatography using epitope specific peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation. -
Tissue specificity
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver. -
Sequence similarities
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Domain
PTB domain mediates receptor interaction. -
Post-translational
modificationsOn immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. - Information by UniProt
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Database links
- Entrez Gene: 9046 Human
- Omim: 604997 Human
- SwissProt: O60496 Human
- Unigene: 71215 Human
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Alternative names
- Docking protein 2 56kDa antibody
- Docking protein 2 antibody
- DOK 2 antibody
see all
Images
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Anti-DOK2 antibody (ab131488) at 1/500 dilution + Jurkat cell extract
Predicted band size: 45 kDa -
Immunofluorescent analysis of methanol-fixed HeLa cells labelling DOK2 with ab131488 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling DOK2 with ab131488 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131488 has not yet been referenced specifically in any publications.