Overview

  • Product name

  • Description

    Rabbit polyclonal to DOK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human DOK2 (internal sequence). The exact sequence is proprietary.
    Database link: O60496

  • Positive control

    • WB: HEK-293T, Jurkat, Raji and NCI-H929 whole cell extracts. ICC/IF: Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 45 kDa.

Target

  • Function

    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
  • Tissue specificity

    Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
  • Sequence similarities

    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain

    PTB domain mediates receptor interaction.
  • Post-translational
    modifications

    On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
  • Information by UniProt
  • Database links

  • Alternative names

    • Docking protein 2 56kDa antibody
    • Docking protein 2 antibody
    • DOK 2 antibody
    • DOK R antibody
    • DOK2 antibody
    • DOK2_HUMAN antibody
    • Downstream of tyrosine kinase 2 antibody
    • OTTHUMP00000161710 antibody
    • OTTHUMP00000224923 antibody
    • p56(dok 2) antibody
    • p56(dok-2) antibody
    • p56dok 2 antibody
    • p56DOK antibody
    • p56dok2 antibody
    see all

Images

  • Anti-DOK2 antibody (ab227184) at 1/10000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-DOK2 antibody (ab227184) at 1/10000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for DOK2 (green) using ab227184 at 1/500 dilution in ICC/IF.

    Nuclear Counterstain: Hoechst 33342 (blue).

References

ab227184 has not yet been referenced specifically in any publications.

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