Key features and details
- Goat polyclonal to DOK3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DOK3 antibody
See all DOK3 primary antibodies
DescriptionGoat polyclonal to DOK3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Human PBMC lysate.
General notesGenBank Accession Number – NP_079148.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab1357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution >1/32000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
Tissue specificityExpressed in spleen.
Sequence similaritiesBelongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
DomainPTB domain mediates receptor interaction.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
Cellular localizationCytoplasm. Cell membrane.
- Information by UniProt
- Docking protein 3 antibody
- DOK 3 antibody
- DOK Like Protein antibody
Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg
Predicted band size: 57 kDa
ab1357 staining (1Primary incubated for 1 hour. Detected by chemiluminescence.
µg/ml) of Human PBMC lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
All lanes : Anti-DOK3 antibody (ab1357) at 2 µg/ml
Lane 1 : Human Bone Marrow lysates
Lane 2 : Peripheral blood leukocyte lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 57 kDa
ab1357 has been referenced in 2 publications.
- Bounab Y et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . PubMed: 23820730
- Heckel T et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . PubMed: 19164586