Key features and details
- Rabbit polyclonal to DOK3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DOK3 antibody
See all DOK3 primary antibodies
DescriptionRabbit polyclonal to DOK3
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human DOK3 aa 1-146.
Database link: Q7L591
- WB: Mouse heart and placenta tissue lysate. IHC-P: Human tonsil tissue. ICC/IF: A549 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000.|
|ICC/IF||1/50 - 1/200.|
FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
Tissue specificityExpressed in spleen.
Sequence similaritiesBelongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
DomainPTB domain mediates receptor interaction.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
Cellular localizationCytoplasm. Cell membrane.
- Information by UniProt
- Docking protein 3 antibody
- DOK 3 antibody
- DOK Like Protein antibody
All lanes : Anti-DOK3 antibody (ab236609) at 1/500 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse placenta tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Paraffin-embedded human tonsil tissue stained for DOK3 using ab236609 at 1/100 dilution in immunohistochemical analysis.
A549 (Human lung carcinoma cell line) cells labeling DOK3 using ab236609 at 1/100 dilution in ICC/IF. The secondary antibody was an Alexa-Fluor®488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236609 has not yet been referenced specifically in any publications.