Overview

  • Product name

  • Description

    Rabbit polyclonal to DOK3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human DOK3 aa 1-146.
    Database link: Q7L591

  • Positive control

    • WB: Mouse heart and placenta tissue lysate. IHC-P: Human tonsil tissue. ICC/IF: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000.
ICC/IF 1/50 - 1/200.

Target

  • Function

    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
  • Tissue specificity

    Expressed in spleen.
  • Sequence similarities

    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain

    PTB domain mediates receptor interaction.
  • Post-translational
    modifications

    Constitutively tyrosine-phosphorylated.
    On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
  • Cellular localization

    Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Docking protein 3 antibody
    • DOK 3 antibody
    • DOK Like Protein antibody
    • Dok3 antibody
    • DOK3_HUMAN antibody
    • DOKL Pending antibody
    • Downstream of tyrosine kinase 3 antibody
    • p62 DOK Like Protein antibody
    see all

Images

  • All lanes : Anti-DOK3 antibody (ab236609) at 1/500 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse placenta tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human tonsil tissue stained for DOK3 using ab236609 at 1/100 dilution in immunohistochemical analysis.

  • A549 (Human lung carcinoma cell line) cells labeling DOK3 using ab236609 at 1/100 dilution in ICC/IF. The secondary antibody was an Alexa-Fluor®488-conjugated goat anti-rabbit IgG (H+L).

References

ab236609 has not yet been referenced specifically in any publications.

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