Recombinant Anti-DOK5 antibody [EPR9923(B)] (ab168343)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9923(B)] to DOK5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DOK5 antibody [EPR9923(B)]
See all DOK5 primary antibodies -
Description
Rabbit monoclonal [EPR9923(B)] to DOK5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human DOK5.
Database link: Q9P104 -
Positive control
- IM9, Human fetal brain and Human fetal heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9923(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab168343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
Target
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Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation. -
Tissue specificity
Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes. -
Sequence similarities
Belongs to the DOK family. Type B subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Domain
PTB domain mediates receptor interaction. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF. - Information by UniProt
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Database links
- Entrez Gene: 55816 Human
- Entrez Gene: 76829 Mouse
- Entrez Gene: 502694 Rat
- Omim: 608334 Human
- SwissProt: Q9P104 Human
- SwissProt: Q91ZM9 Mouse
- Unigene: 656582 Human
- Unigene: 41633 Mouse
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Alternative names
- chromosome 20 open reading frame 180 antibody
- Docking protein 5 antibody
- DOK 5 antibody
see all
Images
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All lanes : Anti-DOK5 antibody [EPR9923(B)] (ab168343) at 1/10000 dilution
Lane 1 : IM9 lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 35 kDa
Datasheets and documents
References (0)
ab168343 has not yet been referenced specifically in any publications.