Key features and details
- Rabbit polyclonal to DOK6
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DOK6 antibody
DescriptionRabbit polyclonal to DOK6
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from an internal sequence within human DOK6.
- COLO cell extracts. A549 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||1/500 - 1/1000.|
RelevanceDOK6 (docking protein 6) is a novel member of the DOK4/5 subclass of the p62 DOK family of intracellular adaptor molecules. DOK6 is highly expressed in the developing central nervous system and is co expressed with Ret in several locations, including sympathetic, sensory, and parasympathetic ganglia, as well as in the ureteric buds of the developing kidneys. Pull down assays using the DOK6 phosphotyrosine binding (PTB) domain and GDNF activated Ret indicate that DOK6 binds to the phosphorylated Ret Tyr(1062) residue. Moreover, ligand activation of Ret resulted in phosphorylation of tyrosine residue(s) located within the unique C terminus of DOK6 predominantly through a Src dependent mechanism, indicating that DOK6 is a substrate of the Ret Src signaling pathway.
- Docking protein 5 antibody
- Docking protein 5 like antibody
- Docking protein 6 antibody
All lanes : Anti-DOK6 antibody (ab72730) at 1/500 dilution
Lane 1 : COLO cell extract
Lane 2 : COLO cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
ab72730 at 1/500 dilution staining DOK6 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab72730 has not yet been referenced specifically in any publications.