Key features and details
- Rabbit polyclonal to DOK7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DOK7 antibody
DescriptionRabbit polyclonal to DOK7
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide derived from N-terminus of human DOK7.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionProbable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
Tissue specificityPreferentially expressed in skeletal muscle and heart Present in thigh muscle, diaphragm and heart but not in the liver or spleen (at protein level).
Involvement in diseaseDefects in DOK7 are the cause of familial limb-girdle myasthenia autosomal recessive (LGM) [MIM:254300]; also called congenital myasthenic syndrome type 1B or CMS1B. LGM is a congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
Sequence similaritiesContains 1 IRS-type PTB domain.
Contains 1 PH domain.
Cellular localizationCell membrane. Cell junction > synapse. Accumulates at neuromuscular junctions.
- Information by UniProt
- Docking protein 7 antibody
- DOK 7 antibody
- DOK7 antibody
All lanes : Anti-DOK7 antibody (ab75049) at 1/500 dilution
Lane 1 : extracts from mouse brain cells
Lane 2 : extracts from mouse brain cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 46 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Human brain tissue with ab75049 at 1/50 dilution, in the absence and presence of the immunising peptide.
HepG2 cells with ab75049 at 1/500 dilution, in the absence and presence of the immunising peptide.
ab75049 has been referenced in 1 publication.
- Zhao H et al. DOK7V1 influences the malignant phenotype of lung cancer cells through PI3K/AKT/mTOR and FAK/paxillin signaling pathways. Int J Oncol 54:381-389 (2019). PubMed: 30431081