Product nameDomoic acid, Kainate agonist
DescriptionKainate agonist. Neurotoxin.
Neurotoxic phycotoxin. Potent agonist at kainate receptors.
Chemical name(2S,3S,4R,5'R)-2-Carboxy-4-(5'-carboxy-1'-methyl-1Z,3E- hexadienyl)-3-pyrrolidineacetic acid
Storage instructionsStore at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions.
Solubility overviewSoluble in water to 25 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab76003 staining MMP9 in U87-MG cells treated with domoic acid (ab120338), by ICC/IF. Increase of MMP9 expression correlates with increased concentration of domoic acid, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120338 (domoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76003 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Adams AL et al. Persistent changes in learning and memory in rats following neonatal treatment with domoic acid. Physiol Behav 96:505-12 (2009). Read more (PubMed: 19146865) »
- Chandrasekaran A et al. Domoic acid-induced neurotoxicity in the hippocampus of adult rats. Neurotox Res 6:105-17 (2004). Read more (PubMed: 15325963) »
- Hampson DR & Manalo JL The activation of glutamate receptors by kainic acid and domoic acid. Nat Toxins 6:153-8 (1998). Read more (PubMed: 10223631) »