Overview

  • Product name

    Donkey Anti-Chicken IgY H&L (FITC)
    See all IgY secondary antibodies
  • Host species

    Donkey
  • Target species

    Chicken
  • Tested applications

    Suitable for: IHC-Frmore details
  • Immunogen

    Purified chicken IgY isolated from normal chicken egg yolks.

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein L purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.

Images

  • ab13970 staining GFP in E10.5 mouse embryo sections by Immunohistochemistry (IHC-Fr - frozen sections).

    Tissue was fixed with paraformaldehyde, permeabilized with PBS/0.5% Triton-X100 and blocked with protein block for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500) for 3 hours at 25°C. FITC-conjugated donkey anti-chicken IgY H&L (ab63507) (1/500) was used as the secondary antibody.

    See Abreview

  • ab63507 was used at dilution 1/100 with the primary antibody ab13970 in IHC - Free Floating. See the review on ab13970.

References

This product has been referenced in:

See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Cryostat sections of E10.5 mouse embryo, which were fixed with PFA before gelatin/sucrose embedding.
Antigen retrieval is optional in this staining.
Primary antibody: Anti-GFP antibody (ab13970) 1:800 at 4 degree overnight.
Secondary antibody: Donkey Anti-Chicken (FITC) ab63507 at RT 4h (long incubation time of secondary would help to increase the signal).

Abcam user community

Verified customer

Submitted Feb 25 2014

Answer

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numerous specific published references. The list for these 4 antibodies is given below:
1. GFAP ab4674 (https://www.abcam.com/gfap-antibody-astrocyte-marker-ab4674.html)
2. FOX3 ab104225 (https://www.abcam.com/fox3neun-antibody-ab104225.html)
3. Iba1 ab5076 (https://www.abcam.com/iba1-antibody-ab5076.html)
4. CD68 ab31630 (https://www.abcam.com/cd68-antibody-ed1-ab31630.html)

I also compiled anti-chicken, rabbit, goat, and mouse secondaries with separate emission spectra. We don't have secondaries for these species that weren't raised in any of the 4 species conjugated to a fluorescent protein with a similar spectrum to DAPI. Thus, I think your best bet is to obtain a far-red filter (Cy5), that way you could still stain with DAPI as well. The donkey secondaries that I found with separate emission spectra are listed below:
1. anti-chicken: FITC (https://www.abcam.com/chicken-igy-secondary-antibody-ab63507.html)
2. anti-rabbit: DyLight 594 (Similar spectrum to Texas Red) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-rabbit-igg-h-l-dylight-594-pre-adsorbed-ab96921.html)
3. anti-goat: DyLight 650 (Similar spectrum to Cy5) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-goat-igg-h-l-dylight-650-pre-adsorbed-ab96938.html)
4. anti-mouse: DyLight 550 (similar spectrum to TRITC) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-mouse-igg-h-l-dylight-550-ab96876.html)

The other antibodies you chose (Nogo, heavy neurofilament, caspase 3) seem to be good choices as well as they are tested in formaldehyde fixed, frozen sections via IHC in rat. Below are the antibodies we discussed against those three proteins:
1. anti-Nogo (https://www.abcam.com/nogo-antibody-ab32298.html)
2. anti-heavy neurofilament (https://www.abcam.com/200-kd-neurofilament-heavy-antibody-ab134459.html)
3. anti-caspase 3 (https://www.abcam.com/active-caspase-3-antibody-ab13847.html)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More
Application
Immunohistochemistry free floating

Sample: Mouse Tissue sections (Brain and spinal cord)
Specification: Brain and spinal cord

Other product details
Dilution: 1/500
Incubation time: 12 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody
Secondary antibody: None used

Additional data
Additional Notes: Please note that the below average score represents a score for an application that was not under the tested applications list on the information sheet.
Abcam response: Thank you for submitting feedback on this antibody in an untested application.

Abcam user community

Verified customer

Submitted Nov 06 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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