Overview

  • Product name

    Donkey F(ab) Anti-Goat IgG H&L
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum. No reaction was observed against anti-Pepsin or anti-Donkey IgG F(c).
  • Tested applications

    Suitable for: ELISA, WB, ICC/IFmore details
  • Immunogen

    Goat IgG whole molecule

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
ICC/IF
  • Application notes
    Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab6520 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question
    Answer

    Thank you for your recent telephone enquiry. This Fab fragment of polyclonal Donkey anti Goat does not have a uniform sequence and I am sorry this has not been sequenced. The molecular weight of Fab fragments is known to be approximately 50 kD non reduced, 25 kD reduced. The generally accepted extinction coefficient at A 280 for these fragments is 1.53. I hope this information is helpful. Please do not hesitate to contact us should you have any further questions.

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