Overview

  • Product name

    Donkey F(ab) Anti-Goat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum. No reaction was observed against anti-Pepsin or anti-Donkey IgG F(c).
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, ELISAmore details
  • Immunogen

    Goat IgG whole molecule

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Donkey IgG Fab
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Flow Cyt
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.


    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab6521 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab6521.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up