Donkey F(ab) Anti-Goat IgG H&L (HRP) (ab6667)

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Overview

  • Product name

    Donkey F(ab) Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Goat

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).

  • Tested applications

    Suitable for: WB, Dot blot, ELISA, Electron Microscopy, IHC, ICC/IFmore details

  • Immunogen

    Goat IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

  • Conjugation notes

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
Dot blot Use at an assay dependent dilution.
ELISA 1/17000. This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy Use at an assay dependent dilution.
IHC Use at an assay dependent dilution.
ICC/IF 1/500 - 1/2500.

References

This product has been referenced in:

  • Li BY  et al. High expression of RAB38 promotes malignant progression of pancreatic cancer. Mol Med Rep 19:909-918 (2019). Read more (PubMed: 30569114) »
  • Büttner-Mainik A  et al. Production of biologically active recombinant human factor H in Physcomitrella. Plant Biotechnol J 9:373-83 (2011). Read more (PubMed: 20723134) »
See all 2 Publications for this product

Publishing research using ab6667? Please let us know so that we can cite the reference in this datasheet.

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