Donkey F(ab) Anti-Goat IgG H&L (HRP) (ab6667)

Submit a review Submit a question References (3)

Key features and details

  • Donkey F(ab) Anti-Goat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: WB, Dot blot, ELISA, Electron Microscopy, IHC, ICC/IF

Overview

  • Product name

    Donkey F(ab) Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Goat

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).

  • Tested applications

    Suitable for: WB, Dot blot, ELISA, Electron Microscopy, IHC, ICC/IFmore details

  • Immunogen

    Goat IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

  • Conjugation notes

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000.
Dot blot
Use at an assay dependent dilution.
ELISA
1/17000. This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy
Use at an assay dependent dilution.
IHC
Use at an assay dependent dilution.
ICC/IF
1/500 - 1/2500.
Notes
WB
1/2000 - 1/10000.
Dot blot
Use at an assay dependent dilution.
ELISA
1/17000. This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy
Use at an assay dependent dilution.
IHC
Use at an assay dependent dilution.
ICC/IF
1/500 - 1/2500.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab6667? Please let us know so that we can cite the reference in this datasheet.

ab6667 has been referenced in 3 publications.

  • Marsala M  et al. Spinal parenchymal occupation by neural stem cells after subpial delivery in adult immunodeficient rats. Stem Cells Transl Med 9:177-188 (2020). PubMed: 31800978
  • Li BY  et al. High expression of RAB38 promotes malignant progression of pancreatic cancer. Mol Med Rep 19:909-918 (2019). PubMed: 30569114
  • Büttner-Mainik A  et al. Production of biologically active recombinant human factor H in Physcomitrella. Plant Biotechnol J 9:373-83 (2011). PubMed: 20723134

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab6667.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com