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Immunology Immunoglobulins Heavy Chain IgG
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Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150138)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150138)
  • Alexa Fluor® - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150138)

Key features and details

  • Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

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Overview

  • Product name

    Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    Reacts with the heavy and light chains of goat IgG as demonstrated by ELISA. May also react with other species and with the light chains of other goat immunoglobulins. Minimal cross reaction to rat and mouse serum proteins.
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity


    Mouse, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), 68% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using chicken, Human, mouse, rabbit and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Fluorophore
    • Alexa Fluor® 555

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab150138 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF (1)
    1/200 - 1/1000.
    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000.
    Notes
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/200 - 1/1000.
    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000.

    Images

    • Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150138)
      Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150138)

      ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150138, Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (yellow) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      The secondary antibody only control displays negative staining, as expected.

    • Alexa Fluor® - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150138)
      Alexa Fluor® - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150138)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab150138? Please let us know so that we can cite the reference in this datasheet.

    ab150138 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    2ND AB-555

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    HAD VERY LESS BACKGROUND STAINING

    Abcam user community

    Verified customer

    Submitted Sep 15 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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