Key features and details
- Donkey F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 647), pre-adsorbed
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Donkey
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, ELISA, IHC-P
Product nameDonkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed
See all IgG secondary antibodies
DescriptionDonkey F(ab')2 polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 647), pre-adsorbed
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
Tested applicationsSuitable for: IHC-Fr, ICC/IF, Flow Cyt, ELISA, IHC-Pmore details
Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
The details of the immunogen for this antibody are not available.
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using chicken, human, mouse, rabbit and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Our Abpromise guarantee covers the use of ab150139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||1/200 - 1/1000.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150139 Alexa Fluor® 647 donkey F(ab)2 anti-goat IgG (H+L) (redas then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
HeLa cells showing negative staining by ICC/IF using only tertiary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.ab150139 Alexa Fluor® 647 donkey F(ab)2 anti-goat IgG (H+L) (redas then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab150139 has been referenced in 2 publications.
- Vanshylla K et al. Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Sci Rep 8:4244 (2018). PubMed: 29523808
- Goertzen C et al. Three-Dimensional Quantification of Spheroid Degradation-Dependent Invasion and Invadopodia Formation. Biol Proced Online 20:20 (2018). PubMed: 30356830