Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab102439)

Overview

  • Product name

    Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Description

    Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.

References

ab102439 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Donkey F(ab)2 anti-hIgG H&L (ab102439) – 1:100 (5ug/ml), 30 min incubation in 4°C.

PE donkey F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal and displaying minimal non-specific binding the cells alone, non-treated with remicade.

Abcam user community

Verified customer

Submitted Apr 25 2017

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