Key features and details
- Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Donkey
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
Product nameDonkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Human IgG whole molecule
ConjugationPE. Ex: 488nm, Em: 575nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Our Abpromise guarantee covers the use of ab7005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/50 - 1/200.|
|IHC-Fr||1/50 - 1/200.|
|Immunomicroscopy||1/50 - 1/200.|
|Flow Cyt||1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.Lesser amounts of reagent may be sufficient for staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7005 has been referenced in 1 publication.
- Zhang Y et al. Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes. Int J Mol Med 41:77-86 (2018). PubMed: 29115390