Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005)

Overview

  • Product name
    Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Human
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Human IgG whole molecule

  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notes
    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality
    Polyclonal
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.

References

This product has been referenced in:
  • Zhang Y  et al. Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes. Int J Mol Med 41:77-86 (2018). Read more (PubMed: 29115390) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means only F(ab')2 portion of the antibody will bind to epitopes of antigen including epitopes on the Fc portion. There is no binding to Fc receptors existing on cell surfaces. The antibody conjugated to PE is extensively cross adsorbed against serum proteins from 10 different species. Each cross that is performed removes more and more reactive antibodies from the pool until a much more narrow collection of antibody remains. While this is great for multiple label experiments, ie. to lower background against horse or goat or rabbit etc., it tends to lower the reactivity of the reagent against some specific epitopes of multiple-epitope antigen and some of the human subclass monoclonals too. I hope this information helps, please do not hesitate to contact us if you need further details,

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Answer

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means only F(ab')2 portion of the antibody will bind to epitopes of antigen including epitopes on the Fc portion. There is no binding to Fc receptors existing on cell surfaces. The antibody conjugated to PE is extensively cross adsorbed against serum proteins from 10 different species. Each cross that is performed removes more and more reactive antibodies from the pool until a much more narrow collection of antibody remains. While this is great for multiple label experiments, ie. to lower background against horse or goat or rabbit etc., it tends to lower the reactivity of the reagent against some specific epitopes of multiple-epitope antigen and some of the human subclass monoclonals too. I hope this information helps, please do not hesitate to contact us if you need further details,

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Answer

Thank you for your enquiry. The concentration of this product is 0.5 mg/mL. The total would be 500ug.

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Answer

The Phycobiliprotein concentration of ab7005 is 0.5mg/mL. For both ab7005 and ab7006, there is one PE molecule with at least one molecule of active antibody. If you have any more questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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