Key features and details
- Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, Flow Cyt, ELISA, IHC-P, ICC/IF
Product nameDonkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
See all IgG secondary antibodies
SpecificityReacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM.
Tested applicationsSuitable for: IHC-Fr, Flow Cyt, ELISA, IHC-P, ICC/IFmore details
Human, Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
The details of the immunogen for this antibody are not available.
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesGel filtration chromatography of pepsin digested antibody.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Our Abpromise guarantee covers the use of ab181347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/200 - 1/1000.|
ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilzed with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal Donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab181347 Alexa Fluor® 647 Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab181347 has been referenced in 3 publications.
- Pellefigues C et al. Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses toNippostrongylus brasiliensis. Front Immunol 8:1575 (2017). PubMed: 29201030
- Georgiades P et al. The flexibility and dynamics of the tubules in the endoplasmic reticulum. Sci Rep 7:16474 (2017). PubMed: 29184084
- Ku T et al. Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues. Nat Biotechnol 34:973-81 (2016). PubMed: 27454740