Product nameDonkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed
See all IgG secondary antibodies
SpecificityReacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins.
Tested applicationsSuitable for: WBmore details
Human, Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
The details of the immunogen for this antibody are not available.
ConjugationAlexa Fluor® 750. Ex: 749nm, Em: 775nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesGel filtration chromatography of pepsin digested antibody.
We batch test Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed, ab175730 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed, ab175730 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Our Abpromise guarantee covers the use of ab175730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.|
Anti-beta Tubulin antibody - Loading Control (ab6046) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175730) at 1/10000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175730 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab175730 has not yet been referenced specifically in any publications.