Product nameDonkey F(ab')2 Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-Pmore details
Chicken, Cow, Goat, Horse, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
ConjugationDyLight® 488. Ex: 493nm, Em: 518nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
Our Abpromise guarantee covers the use of ab98488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/200.|
|ICC/IF||1/50 - 1/500.|
|IHC-P||1/50 - 1/500.|
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin a1 (basement membrane marker), 1:400
Secondary antibody: Donkey F(ab')2 polyclonal to Rabbit IgG - H&L DyLight® 488 (ab98488), 1:200
F-actin was stained with rhodamine-phallodoin 1:100
Nuclei were counterstained with DAPI
Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
This product has been referenced in:
- Yuan YH et al. Epigenetic inactivation of HOXD10 is associated with human colon cancer via inhibiting the RHOC/AKT/MAPK signaling pathway. Cell Commun Signal 17:9 (2019). Read more (PubMed: 30683109) »
- Qin D et al. Ultrasound-targeted microbubble destruction-mediated miR-205 enhances cisplatin cytotoxicity in prostate cancer cells. Mol Med Rep 18:3242-3250 (2018). Read more (PubMed: 30066866) »