Donkey F(ab')2 Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab98488)

Overview

  • Product name

    Donkey F(ab')2 Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Mouse, Rat, Sheep more details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin a1 (basement membrane marker), 1:400

    Secondary antibody: Donkey F(ab')2 polyclonal to Rabbit IgG - H&L DyLight® 488 (ab98488), 1:200

    F-actin was stained with rhodamine-phallodoin 1:100
    Nuclei were counterstained with DAPI
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Yuan YH  et al. Epigenetic inactivation of HOXD10 is associated with human colon cancer via inhibiting the RHOC/AKT/MAPK signaling pathway. Cell Commun Signal 17:9 (2019). Read more (PubMed: 30683109) »
  • Qin D  et al. Ultrasound-targeted microbubble destruction-mediated miR-205 enhances cisplatin cytotoxicity in prostate cancer cells. Mol Med Rep 18:3242-3250 (2018). Read more (PubMed: 30066866) »
See all 8 Publications for this product

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