Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
Key features and details
- Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Immunomicroscopy, Flow Cyt
Overview
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Product name
Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
See all IgG secondary antibodies -
Description
Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Immunomicroscopy, Flow Cytmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Rabbit IgG whole molecule
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Conjugation
PE. Ex: 488nm, Em: 575nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins -
Conjugation notes
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab7007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/200. | |
ICC/IF | 1/100 - 1/250. | |
Immunomicroscopy | Use at an assay dependent dilution. | |
Flow Cyt | 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (19)
ab7007 has been referenced in 19 publications.
- Amjadi F et al. Distinct changes in the proteome profile of endometrial tissues in polycystic ovary syndrome compared with healthy fertile women. Reprod Biomed Online N/A:N/A (2018). WB, IHC-P . PubMed: 29729850
- Vasilev DS et al. Transient Morphological Alterations in the Hippocampus After Pentylenetetrazole-Induced Seizures in Rats. Neurochem Res N/A:N/A (2018). PubMed: 29936569
- Xu YL et al. MicroRNA-19 restores vascular endothelial cell function in lower limb ischemia-reperfusion injury through the KLF10-dependent TGF-ß1/Smad signaling pathway in rats. J Cell Biochem N/A:N/A (2018). PubMed: 29953651
- da Silva Lima F et al. An insight into the role of magnesium in the immunomodulatory properties of mesenchymal stem cells. J Nutr Biochem 55:200-208 (2018). Flow Cyt, ICC/IF . PubMed: 29554498
- Vaez SA et al. The cardiac niche role in cardiomyocyte differentiation of rat bone marrow-derived stromal cells: comparison between static and microfluidic cell culture methods. EXCLI J 17:762-774 (2018). PubMed: 30190666