Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)

Submit an Abreview Q&A (4)Specific References (14)

Overview

  • Product name
    Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Immunomicroscopy, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prepared from monospecific antiserum by immunoaffinity chromatography using rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins
  • Conjugation notes
    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
ICC/IF 1/100 - 1/250.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.

Images

  • Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
    Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
    Ab7007 was used with the primary antibody ab18203 in Flow Cyt. See Abreview on ab18203.
  • Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
    Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
    Ab7007 was used at dilution 1/250 with the primary antibody ab24182 in ICC/IF. See the review on ab24182.

References

This product has been referenced in:
  • Haratizadeh S  et al. Condition medium of cerebrospinal fluid and retinoic acid induces the transdifferentiation of human dental pulp stem cells into neuroglia and neural like cells. Anat Cell Biol 50:107-114 (2017). Read more (PubMed: 28713614) »
  • Harwood SL  et al. ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy. Oncoimmunology 7:e1377874 (2017). Flow Cyt . Read more (PubMed: 29296540) »

See all 14 Publications for this product

Publishing research using ab7007? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

10
Votes: Highest First

Customer kindly contacted us to inquire about the immunogen sequence for ab56215.

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is ...

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High FACS signal from secondary-only control. What modification do you suggest?

Thank you for your reply. Your protocol looks correct. The only possible issues I see are the concentration of the secondary antibody that you incubate with the cells but more importantly, the lack of saponin in the wash buffer. The saponin permeabiliz...

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Dear Sirt’s Reference to the above subject (Glucocorticoid Receptor; ab9568 or ab109022) .. the customer need to work it by Flow Cytometer.. and I think this type of work need a secondary anti-body. But the TDS (technical data sheet) on the abcam websi...

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Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - ra...

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Please confirm that this antibody has been tested for application in IHC-frozen sections, and if yes, what dilution is recommended.

Thank you for your phone call. I checked with the originator of this antibody and they informed me that it was not specifically tested for application in IHC-frozen sections or formalin-fixed paraffin embedded sections. I have updated our datasheet to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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