Donkey Anti-Goat IgG H&L (18nm Gold) preadsorbed (ab105270)

Submit a review Q&A (3)References (2)

Key features and details

  • Donkey Anti-Goat IgG H&L (18nm Gold) preadsorbed
  • Conjugation: Gold 18nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: Electron Microscopy, ELISA, WB

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (18nm Gold) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Goat

  • Specificity

    Recognizes the heavy chains on goat IgG and the light chains common to most goat immunoglobulins. Does not crossreact with non-immunoglobulin serum proteins. Exhibits minimal crossreactivity to chicken, guinea pig, hamster, horse, human, mouse, rabbit and rat serum proteins.

  • Tested applications

    Suitable for: Electron Microscopy, ELISA, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat more details

  • Conjugation

    Gold 18nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 8.50
    Preservative: 0.05% Sodium azide
    Constituents: 1.5% BSA, 0.87% Sodium chloride, 0.164% Sodium phosphate, 0.381% Sodium borate
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy 1/10 - 1/20.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab105270? Please let us know so that we can cite the reference in this datasheet.

ab105270 has been referenced in 2 publications.

  • Asadi F & Dhanvantari S Stathmin-2 Mediates Glucagon Secretion From Pancreatic a-Cells. Front Endocrinol (Lausanne) 11:29 (2020). PubMed: 32117057
  • Rathsam C  et al. Characterization of inter-crystallite peptides in human enamel rods reveals contribution by the Y allele of amelogenin. J Struct Biol 204:26-37 (2018). PubMed: 29959991

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

For our future work, we would need a per-deuterated secondary antibody.
Do you know if you guys have something like that or would know where to
buy such a deuterated antibody?
Thanks!

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Answer

Thank you for contacting us.

We do not have per-deuterated antibodies. You can search in the biocompare website at http://www.biocompare.com.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

concentration d'ab105270, lot GR106147-1.

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Answer

J’ai téléphoné au laboratoire cet après-midi qui m’a dit que nous n'avons pas déterminé la concentration de cet anticorps.

Nous ne savons pas la concentration des anticorps complexé à l’or parce que nous séparons les monomères des multimères sur des gradients de densité. Nous n'avons aucun moyen fiable de mesurer la concentration d'anticorps sur les monomères parce que la préparation est stabilisée avec de l'albumine avant et après centrifugation.

Nous vous suggérons d'utiliser une dilution 1:10 ou 1:20 pour la microscopie électronique.

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Question

Dear Technical Department,
I would like to know the concentration of the actual antibody in the
as-received solution. please see below for the specific order in
question.
Thanks in advance for the info!

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Answer

Thank you for contacting us.

We do not have concentration levels for this Gold labeled antibody. We do not know the antibody concentration in gold complexes because we separate monomers from multimers on density gradients. We do not have any reliable way to measure the antibody concentration on the monomers because the preparation is stabilized with albumin before and after centrifugation.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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