Donkey Anti-Goat IgG H&L (ab182021)
- Datasheet
- References
- Protocols
Overview
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Product name
Donkey Anti-Goat IgG H&L
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Specificity
The antibody reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, mouse IgG, rat IgG, rabbit IgG and chicken IgY, less than 2%. -
Tested applications
Suitable for: WB, IHC-P, IP, ELISAmore details -
Immunogen
Full length protein corresponding to Goat IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
Our Abpromise guarantee covers the use of ab182021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| ELISA | 1/20000 - 1/200000. |
Images
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ELISA - Donkey Anti-Goat IgG H&L (ab182021)Cross-reactivity of the polyclonal secondary antibody ab182021 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182021 was then added starting at 1 µg/ml and gradually diluted 1:4 (50µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1:20,000 dilution (50µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182021 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, rabbit IgG and chicken IgY.
Datasheets and documents
References
ab182021 has not yet been referenced specifically in any publications.
