Key features and details
- Donkey Anti-Goat IgG H&L
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, IHC-P, IP, ELISA
Product nameDonkey Anti-Goat IgG H&L
See all IgG secondary antibodies
SpecificityThe antibody reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, mouse IgG, rat IgG, rabbit IgG and chicken IgY, less than 2%.
Tested applicationsSuitable for: WB, IHC-P, IP, ELISAmore details
Full length protein corresponding to Goat IgG.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
Purification notesImmunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab182021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ELISA||1/20000 - 1/200000.|
Cross-reactivity of the polyclonal secondary antibody ab182021 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182021 was then added starting at 1 µg/ml and gradually diluted 1:4 (50µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1:20,000 dilution (50µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182021 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, rabbit IgG and chicken IgY.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab182021 has not yet been referenced specifically in any publications.