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  1. Link

    donkey-goat-igg-hl-ab182021.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Goat IgG H&L (ab182021)

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  • ELISA - Donkey Anti-Goat IgG H&L (ab182021)
  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Donkey Anti-Goat IgG H&L
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    The antibody reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, mouse IgG, rat IgG, rabbit IgG and chicken IgY, less than 2%.
  • Tested applications

    Suitable for: WB, IHC-P, IP, ELISAmore details
  • Immunogen

    Full length protein corresponding to Goat IgG.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Unconjugated

Applications

Our Abpromise guarantee covers the use of ab182021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA 1/20000 - 1/200000.

Images

  • ELISA - Donkey Anti-Goat IgG H&L (ab182021)
    ELISA - Donkey Anti-Goat IgG H&L (ab182021)

    Cross-reactivity of the polyclonal secondary antibody ab182021 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182021 was then added starting at 1 µg/ml and gradually diluted 1:4 (50µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1:20,000 dilution (50µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182021 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, rabbit IgG and chicken IgY.

Datasheets and documents

    • Datasheet
  • References

    ab182021 has not yet been referenced specifically in any publications.

    Publishing research using ab182021? Please let us know so that we can cite the reference in this datasheet.

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