Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175664)
- Datasheet
- References (11)
- Protocols
Overview
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Product name
Donkey Anti-Goat IgG H&L (Alexa Fluor® 405)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Specificity
This antibody is specific to Goat IgG -
Tested applications
Suitable for: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-Pmore details -
Immunogen
Full length protein corresponding to Goat IgG.
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Conjugation
Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab175664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
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| ELISA | Use at an assay dependent concentration. | |
| Flow Cyt | 1/2000. | |
| IHC-P | Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175664)ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab175664 Alexa Fluor® 405 donkey anti-goat IgG (H+L) (blue) was then used at 2µg/ml for 1h as a tertiary antibody. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175664)
Datasheets and documents
References
This product has been referenced in:
- Hsu M et al. Neuroinflammation-induced lymphangiogenesis near the cribriform plate contributes to drainage of CNS-derived antigens and immune cells. Nat Commun 10:229 (2019). Read more (PubMed: 30651548) »
- Corbett BF et al. Sphingosine-1-phosphate receptor 3 in the medial prefrontal cortex promotes stress resilience by reducing inflammatory processes. Nat Commun 10:3146 (2019). Read more (PubMed: 31316053) »
