For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    donkey-goat-igg-hl-alexa-fluor-405-preadsorbed-ab175665.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgG
Share by email

Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
  • Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
  • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)

Key features and details

  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

You may also be interested in

Secondary
Product image
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed (ab175659)
Blocking
Product image
Normal Donkey Serum (ab7475)

View more associated products

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Human, Mouse, Pig, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length protein corresponding to Goat IgG.

  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Fluorophore
    • Alexa Fluor® 405

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab175665 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr (1)
    Use at an assay dependent concentration.
    ICC/IF
    1/200 - 1/1000.

    We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) 
    Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm 
    Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000.
    Notes
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/200 - 1/1000.

    We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) 
    Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm 
    Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000.

    Images

    • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
      Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)

      ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab175665 Alexa Fluor® 405 Donkey polyclonal Secondary Antibody to Goat IgG - H&L pre-adsorbed (blue) was then used at 1µg/ml for 1h as a tertiary antibody. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.

      The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

    • Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
      Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)This image is courtesy of an Abreview submitted by Bryan Niedenberger

      Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B) Goat Anti-DDX4 at a 1/800 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175665) applied at a 1/500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.

    • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
      Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab175665? Please let us know so that we can cite the reference in this datasheet.

    ab175665 has been referenced in 3 publications.

    • Park HE  et al. Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio. Adv Sci (Weinh) 6:1901673 (2019). PubMed: 31763149
    • Strubberg AM  et al. Cftr Modulates Wnt/ß-Catenin Signaling and Stem Cell Proliferation in Murine Intestine. Cell Mol Gastroenterol Hepatol 5:253-271 (2018). PubMed: 29675451
    • Wilson DE  et al. GABAergic Neurons in Ferret Visual Cortex Participate in Functionally Specific Networks. Neuron 93:1058-1065.e4 (2017). PubMed: 28279352

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Excellent results

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    This antibody works well at a 1:500 dilution and gives a bright signal with little discernible nonspecific staining.
    Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B) Goat Anti-DDX4 at a 1:800 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Donkey Anti-Goat 405 (abcam ab175665) applied at a 1:500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.

    Mr. Bryan Niedenberger

    Verified customer

    Submitted May 06 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.