Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133)

Overview

  • Product name
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 488), pre-adsorbed
  • Host species
    Donkey
  • Target species
    Goat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Human, Mouse, Pig, Rabbit, Rat more details
  • Immunogen

    Full length protein corresponding to Goat IgG.

  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/2000.
ELISA Use at an assay dependent concentration.

Images

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150133 Alexa Fluor® 488 donkey anti-goat IgG (H+L) (green) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

References

This product has been referenced in:
  • Lodge EJ  et al. Homeostatic and tumourigenic activity of SOX2+ pituitary stem cells is controlled by the LATS/YAP/TAZ cascade. Elife 8:N/A (2019). Read more (PubMed: 30912742) »
  • Yuasa H  et al. Effects of the expansion of bacterial colonies into the intervillous spaces on the localization of several lymphocyte lineages in the rat ileum. J Vet Med Sci 81:555-566 (2019). Read more (PubMed: 30799326) »
See all 8 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Good

Good
Abreviews
Application
Immunohistochemistry (PFA fixed)
Stain free floating sections of mouse brain. The concentration is 1:250

Abcam user community

Verified customer

Submitted Jul 21 2017

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Asynchronous HeLa were paraformaldehyde-fixed, permeablized, immunofluorescently labeled, counterstained with DAPI and imaged. Under these conditions this antibody gave a very strong signal. MeOH fixed samples were also evaluated and produced an equally strong signal.

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 27 2016

Abreviews
Application
Immunohistochemistry (Frozen sections)
anti-body was diluted 1/250. in the same assay we used abcam primary anti-GFP antibody (ab6673) in dilution of 1/500

Abcam user community

Verified customer

Submitted Aug 21 2014

Answer

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab150129. I would also appreciate if you can confirm some further details: Do you use a detergent such as Tween 20 in your washing buffer? Have you attempted to increase the number and duration of washes after antibody incubation? If so, did this have affect on the background? Have you attempted to further dilute the antibody to 1/1500, 1/2000 or greater? This antibody has not been pre-absorbed against mouse IgG, as such we cannot guarantee that we will be able to eliminate this reactive thought we hope that these suggestions will help reduce this. Abcam does offer a number of AlexaFluor 488 antibodies which have been pre-absorbed to ensure low species cross-reactivity including ab150133 which has been pre-absorbed against chicken, human, mouse, pig, rabbit and rat. More details about this product may be found at: https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-goat-igg-hl-alexa-fluorreg-488-pre-adsorbed-ab150133.html

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