Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Alexa Fluor® 555)
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 555)
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    This antibody is specific to Goat IgG
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Immunogen

    Full length protein corresponding to Goat IgG.

  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/2000.

Images

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150130 Alexa Fluor® 555 donkey anti-goat IgG (H+L) (yellow) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Immunocytochemsitry/Immunfluorescence analysis of mouse brain organotypic slice labelling GFAP primary with Alexa Fluor® 555 Donkey anti-goat IgG H&L (ab150130). Counter stained with the nuclear marker TO-PRO-3. Clear staining shown with 543nm laser.

References

This product has been referenced in:

  • Zheng L  et al. Phosphorylation of Cx43 residue Y313 by Src contributes to blocking the interaction with Drebrin and disassembling gap junctions. J Mol Cell Cardiol 126:36-49 (2019). Read more (PubMed: 30448479) »
  • Lim DW  et al. Chlorogenic Acid from Hawthorn Berry (Crataegus pinnatifida Fruit) Prevents Stress Hormone-Induced Depressive Behavior, through Monoamine Oxidase B-Reactive Oxygen Species Signaling in Hippocampal Astrocytes of Mice. Mol Nutr Food Res N/A:e1800029 (2018). Read more (PubMed: 29893510) »
See all 10 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Very clear staining works well with the 543nm laser on the Zeiss LSM 710 Confocal to give good images. I have used it in combination with the nuclear marker TOPRO - 3.

Ms. Kirsty Ireland

Verified customer

Submitted Dec 09 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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