Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150134)

Overview

  • Product name
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 555), pre-adsorbed
  • Host species
    Donkey
  • Target species
    Goat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-Pmore details
  • Minimal
    cross-reactivity

    Chicken, Human, Mouse, Pig, Rabbit, Rat more details
  • Immunogen

    Full length protein corresponding to Goat IgG.

  • Conjugation
    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using chicken, Human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/2000.
IHC-P Use at an assay dependent concentration.

Images

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150134 Alexa Fluor® 555 donkey anti-goat IgG (H+L) (yellow) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • HeLa cells showing negative staining by ICC/IF using only tertiary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab150134 Alexa Fluor® 555 donkey anti-goat IgG (H+L) (yellow) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Song XB  et al. Autophagy blockade and lysosomal membrane permeabilization contribute to lead-induced nephrotoxicity in primary rat proximal tubular cells. Cell Death Dis 8:e2863 (2017). Read more (PubMed: 28594408) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA fixed)
Rat brain at 3 days after photothrombotic stroke was perfusion-fixed using 4% PFA and cryo-sectioned in the coronal plane (20 µm thick).
Primary antibody: Goat anti-Iba1 antibody (Abcam ab5076)
Secondary antibody: Donkey anti-goat IgG H&L (Alexa Fluor® 555) preadsorbed (Abcam ab150134)
Blocking buffer: 10% Donkey serum, 3% BSA, 0.3% Triton X-100 in PBS (pH 7.4)
Brain section was blocked for 2 h at room temperature.
Incubation in primary antibody diluted in blocking buffer (1:500 dilution) at 4 °C for 16-18 h.
Incubation in secondary antibody diluted in PBS (1:1000 dilution) at room temperature for 2 h.
Image (scale bar = 200 µm) shown is taken from the peri-infarct cortex.
Strong fluorescent signal with moderate background noise can be observed.
Username

Abcam user community

Verified customer

Submitted Nov 18 2016

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Asynchronous HeLa cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, this antibody produces a strong fluorescent signal. MeOH fixed samples were also evaluated and the antibody produced a similar strong fluorescent signal.
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 27 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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