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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
  • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)

Key features and details

  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed
  • Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, IHC-P, ELISA, ICC/IF, Flow Cyt

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Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ELISA, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Human, Mouse, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length protein corresponding to Goat IgG.

  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Fluorophore
    • Alexa Fluor® 568

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab175704 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr Use at an assay dependent concentration.
    IHC-P Use at an assay dependent concentration.
    ELISA Use at an assay dependent concentration.
    ICC/IF 1/200 - 1/1000.
    Flow Cyt 1/2000.

    Images

    • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
      Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)

      ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, Goat anti-Mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab175704 Alexa Fluor® 568 Donkey anti-Goat IgG(H&L) (yellow) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

       

      Alexa Fluor® 568 is pseudocolored in yellow.

    • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
      Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
      HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab175704 Alexa Fluor® 568 Donkey anti-Goat IgG(H&L) (yellow) was used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

       

      Alexa Fluor® 568 is pseudocolored in yellow.

    • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
      Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (10)

    Publishing research using ab175704? Please let us know so that we can cite the reference in this datasheet.

    ab175704 has been referenced in 10 publications.

    • Kim AJ  et al. Endothelial Cell Lineage Analysis Does Not Provide Evidence for EMT in Adult Valve Homeostasis and Disease. Anat Rec (Hoboken) 302:125-135 (2019). PubMed: 30306735
    • Medrano-Jiménez E  et al. Malva parviflora extract ameliorates the deleterious effects of a high fat diet on the cognitive deficit in a mouse model of Alzheimer's disease by restoring microglial function via a PPAR-?-dependent mechanism. J Neuroinflammation 16:143 (2019). PubMed: 31291963
    • Wang H  et al. Decreased levels of superoxide dismutase in inner pillar cells contribute to ribbon synapse impairment in presbycusis. Am J Transl Res 11:2403-2412 (2019). PubMed: 31105846
    • Polpitiya Arachchige S  et al. Analysis of Select Herpes Simplex Virus 1 (HSV-1) Proteins for Restriction of Human Immunodeficiency Virus Type 1 (HIV-1): HSV-1 gM Protein Potently Restricts HIV-1 by Preventing Intracellular Transport and Processing of Env gp160. J Virol 92:N/A (2018). PubMed: 29093081
    • Tsuneoka Y  et al. Neurotransmitters and neuropeptides in gonadal steroid receptor-expressing cells in medial preoptic area subregions of the male mouse. Sci Rep 7:9809 (2017). PubMed: 28852050
    • Hansen JS  et al. Visualization of lipid directed dynamics of perilipin 1 in human primary adipocytes. Sci Rep 7:15011 (2017). IF ; Human . PubMed: 29118433
    • Hedrick T  et al. Characterization of Channelrhodopsin and Archaerhodopsin in Cholinergic Neurons of Cre-Lox Transgenic Mice. PLoS One 11:e0156596 (2016). IHC . PubMed: 27243816
    • Hansen JS  et al. Perilipin 1 binds to aquaporin 7 in human adipocytes and controls its mobility via protein kinase A mediated phosphorylation. Metabolism 65:1731-1742 (2016). PubMed: 27832861
    • Costantini I  et al. A versatile clearing agent for multi-modal brain imaging. Sci Rep 5:9808 (2015). PubMed: 25950610
    • Kim SH  et al. Electrogenic transport and K(+) ion channel expression by the human endolymphatic sac epithelium. Sci Rep 5:18110 (2015). IHC ; Human . PubMed: 26655723

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Donkey Anti-Goat lgG H&L

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Alexa Fluor®
    Control Mouse lung section / secondary antibody dilution 1:2000 , 1 hr / Room temperature / protect from light

    Pureunhaneul Lee

    Verified customer

    Submitted Oct 06 2015

    ab175704 - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed.

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Used at 1 in 1000 for 2 hours at room temperature followed by 3 washes in 0.1% PBS-Triton X. Staining was specific (as confirmed by negative control) and noise was minimal.

    Abcam user community

    Verified customer

    Submitted Apr 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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