Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
Key features and details
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, IHC-P, ELISA, ICC/IF, Flow Cyt
Overview
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Product name
Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: IHC-Fr, IHC-P, ELISA, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Chicken, Human, Mouse, Rabbit, Rat more details -
Immunogen
Full length protein corresponding to Goat IgG.
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Conjugation
Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab175704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. | |
| Flow Cyt | 1/2000. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, Goat anti-Mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab175704 Alexa Fluor® 568 Donkey anti-Goat IgG(H&L) (yellow) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® 568 is pseudocolored in yellow.
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab175704 Alexa Fluor® 568 Donkey anti-Goat IgG(H&L) (yellow) was used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.Alexa Fluor® 568 is pseudocolored in yellow.
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Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175704)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (10)
ab175704 has been referenced in 10 publications.
- Kim AJ et al. Endothelial Cell Lineage Analysis Does Not Provide Evidence for EMT in Adult Valve Homeostasis and Disease. Anat Rec (Hoboken) 302:125-135 (2019). PubMed: 30306735
- Medrano-Jiménez E et al. Malva parviflora extract ameliorates the deleterious effects of a high fat diet on the cognitive deficit in a mouse model of Alzheimer's disease by restoring microglial function via a PPAR-?-dependent mechanism. J Neuroinflammation 16:143 (2019). PubMed: 31291963
- Wang H et al. Decreased levels of superoxide dismutase in inner pillar cells contribute to ribbon synapse impairment in presbycusis. Am J Transl Res 11:2403-2412 (2019). PubMed: 31105846
- Polpitiya Arachchige S et al. Analysis of Select Herpes Simplex Virus 1 (HSV-1) Proteins for Restriction of Human Immunodeficiency Virus Type 1 (HIV-1): HSV-1 gM Protein Potently Restricts HIV-1 by Preventing Intracellular Transport and Processing of Env gp160. J Virol 92:N/A (2018). PubMed: 29093081
- Tsuneoka Y et al. Neurotransmitters and neuropeptides in gonadal steroid receptor-expressing cells in medial preoptic area subregions of the male mouse. Sci Rep 7:9809 (2017). PubMed: 28852050
- Hansen JS et al. Visualization of lipid directed dynamics of perilipin 1 in human primary adipocytes. Sci Rep 7:15011 (2017). IF ; Human . PubMed: 29118433
- Hedrick T et al. Characterization of Channelrhodopsin and Archaerhodopsin in Cholinergic Neurons of Cre-Lox Transgenic Mice. PLoS One 11:e0156596 (2016). IHC . PubMed: 27243816
- Hansen JS et al. Perilipin 1 binds to aquaporin 7 in human adipocytes and controls its mobility via protein kinase A mediated phosphorylation. Metabolism 65:1731-1742 (2016). PubMed: 27832861
- Costantini I et al. A versatile clearing agent for multi-modal brain imaging. Sci Rep 5:9808 (2015). PubMed: 25950610
- Kim SH et al. Electrogenic transport and K(+) ion channel expression by the human endolymphatic sac epithelium. Sci Rep 5:18110 (2015). IHC ; Human . PubMed: 26655723
